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JoVE Journal Developmental Biology
Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
 

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

Article DOI: 10.3791/52910-v • 08:14 min • December 3rd, 2015
December 3rd, 2015 •
1, 1, 2
1Primate Research Center, University of Wisconsin-Madison, 2Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health

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Resumo
Automatic Translation

This protocol describes the efficient induction of hemogenic endothelium and multipotential hematopoietic progenitors from human pluripotent stem cells via the forced expression of transcription factors.

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Hemogenic Endothelium Hematopoietic Cells Pluripotent Stem Cells Transcription Factors ETV2 GATA1 GATA2 TAL1 LMO2 Endothelial-hematopoietic Transition Erythroid Megakaryocytic In Vitro Modeling
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