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In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells
 

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

Article DOI: 10.3791/56096-v • 09:41 min • November 2nd, 2017
November 2nd, 2017 •
*1,2, *1,2, 1,2, 1,2
1Department of Ophthalmology, Eye and ENT Hospital of Fudan University, 2Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University
* These authors contributed equally

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Small ubiquitin-related modifier (SUMO) family proteins are conjugated to the lysine residues of target proteins to regulate various cellular processes. This paper describes a protocol for the detection of retinoblastoma (Rb) protein SUMOylation under endogenous and exogenous conditions in human cells.

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Keywords: Rb Protein SUMOylation In Vivo Detection Human Cells SUMO Conjugation Cell Synchronization G1 Phase S Phase G2/M Phase HEK293 Cells Cell Cycle Analysis Flow Cytometry RIPA Lysis
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