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Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
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JoVE Journal Genética
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

DOI:
10.3791/56187-v

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11:42 min

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October 27, 2017

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, ,
1Division of Endocrinology, Diabetes and Nutrition, Department of Medicine,University of Maryland School of Medicine

Capítulos

  • 00:05Título
  • 01:04Generating Embryos Through Natural Mating and Staging Embryos
  • 02:22Whole Embryo Dissociation, RNA Extraction and Purify High-quality RNA
  • 04:55Sorting Differentially Expressed Genes
  • 07:00Determine Enriched Pathways
  • 07:57Generation of Pathway Networks and Determination of Enriched GO Terms
  • 09:12Results: RNAseq-based Zebrafish Genome Expression Data for Alstrom and Bardet Biedl Syndromes
  • 10:47Conclusion

Summary

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This protocol presents an approach for whole transcriptome analysis from zebrafish embryos, larvae, or sorted cells. We include isolation of RNA, pathway analysis of RNASeq data, and qRT-PCR-based validation of gene expression changes.

Tags

RNASeqGene ExpressionZebrafishEmbryoLarvaTranscriptomeSample PreparationRNA ExtractionRNA SequencingBiologia do DesenvolvimentoComparative Analysis

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