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サンプル準備およびゼブラフィッシュから RNASeq による遺伝子発現データの解析
JoVE Journal
Genética
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JoVE Journal Genética
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
DOI:

11:42 min

October 27, 2017

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Capítulos

  • 00:05Título
  • 01:04Generating Embryos Through Natural Mating and Staging Embryos
  • 02:22Whole Embryo Dissociation, RNA Extraction and Purify High-quality RNA
  • 04:55Sorting Differentially Expressed Genes
  • 07:00Determine Enriched Pathways
  • 07:57Generation of Pathway Networks and Determination of Enriched GO Terms
  • 09:12Results: RNAseq-based Zebrafish Genome Expression Data for Alstrom and Bardet Biedl Syndromes
  • 10:47Conclusion

Summary

Tadução automática

このプロトコルは、ゼブラフィッシュの胚は、幼虫から全体のトランスクリプトーム解析手法を提案またはセルを並べ替えられます。我々 には、RNA の隔離、RNASeq データの経路解析と遺伝子発現変動の qRT PCR による検証。

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