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斑马鱼 RNASeq-based 基因表达数据的样品制备与分析

JoVE Journal
Genética

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JoVE Journal Genética

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

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斑马鱼 RNASeq-based 基因表达数据的样品制备与分析

DOI:

10.3791/56187-v

•

11:42 min

•

October 27, 2017

•

Timothy L. Hostelley*¹, Jessica E. Nesmith*¹, Norann A. Zaghloul

¹Division of Endocrinology, Diabetes and Nutrition, Department of Medicine,University of Maryland School of Medicine

Capítulos

00:05Título
01:04Generating Embryos Through Natural Mating and Staging Embryos
02:22Whole Embryo Dissociation, RNA Extraction and Purify High-quality RNA
04:55Sorting Differentially Expressed Genes
07:00Determine Enriched Pathways
07:57Generation of Pathway Networks and Determination of Enriched GO Terms
09:12Results: RNAseq-based Zebrafish Genome Expression Data for Alstrom and Bardet Biedl Syndromes
10:47Conclusion

Summary

Tadução automática

该协议提出了一种从斑马鱼胚胎, 幼虫, 或排序细胞的整体转录分析的方法。我们包括 RNA 的分离, RNASeq 数据的通路分析, 和 qRT pcr 验证基因表达的变化。

Tags

RNASeq Gene Expression Zebrafish Embryo Larva Transcriptome Sample Preparation RNA Extraction RNA Sequencing Biologia do Desenvolvimento Comparative Analysis

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