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JoVE Journal Genetics
Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning
 

Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning

Article DOI: 10.3791/56319-v • 12:06 min • October 23rd, 2017
October 23rd, 2017 •
1, 1, 1,2,3, 1
1Department of Translational Research, Tsurumi University School of Dental Medicine, 2Endowed Department of International Oral Health Science (affiliated with Department of Translational Research), Tsurumi University School of Dental Medicine, 3Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University

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We describe a facile, rapid, and relatively inexpensive method for the generation of gene-disrupted Streptococcus mutans strains; this technique may be adapted for the generation of gene-disrupted strains of various species.

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Gene-disrupted Streptococcus Mutans Gene Cloning Fusion PCR Electroporation Microbiology Enzymatic Reaction Competent Cell Preparation Streptococcus Mutans Gene Disruption Staphylococcus Aureus Nested Primers Doctor Ayako Okada Research Instructor S.mutans UA159 Brain Heart Infusion BHI Agar Plate Anaerobic Conditions Toothpick BHI Broth Centrifuge PBS
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