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JoVE Journal Bioengineering
Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
 

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

Article DOI: 10.3791/56343-v • 07:27 min • November 1st, 2017
November 1st, 2017 •
1, 2, 3, 2, 1
1Department of Medical Engineering, California Institute of Technology, 2Department of Earth Sciences, University of Southern California, 3Jet Propulsion Laboratory, California Institute of Technology

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Digital holographic microscopy (DHM) is a volumetric technique that allows imaging samples 50-100X thicker than brightfield microscopy at comparable resolution, with focusing performed post-processing. Here DHM is used for identifying, counting, and tracking microorganisms at very low densities and compared with optical density measurements, plate count, and direct count.

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Microorganisms Low Concentrations Digital Holographic Microscopy Sensitivity Real-time Information Bacterial Behaviors Biological Field Cosmological Field Pathogenic Organisms Water Solar System Icy Moons Volumetric Technique Three-dimensional Information Physical Refocus Diagnosis Blood Stream Infections Cerebrospinal Fluid Infections Spectrophotometric Reading Bacterial Master Culture Petroff-Hausser Counting Chamber Live Cells Dead Cells Micropipette High Dry Objective Microscope Phase Contrast
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