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Bioengineering

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JoVE Journal Bioengineering
Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy
 

Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Article DOI: 10.3791/63931-v • 06:37 min • June 15th, 2022
June 15th, 2022 •
1, 2, 2, 3, 3, 2, 2
1Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, 2Department of Physics and Biophysics, University of San Diego, 3Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology

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Differential dynamic microscopy (DDM) combines features of dynamic light scattering and microscopy. Here, the process of using DDM to characterize reconstituted cytoskeleton networks by quantifying the subdiffusive and caged dynamics of particles in vimentin networks and the ballistic motion of active myosin-driven actin-microtubule composites is presented.

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Keywords: Cytoskeleton Dynamics Differential Dynamic Microscopy Reconstituted Cytoskeleton Networks Soft Materials Biologically Relevant Materials Microscope Control Software PyDDM Code Repository DDM Matrix Intermediate Scattering Function Data Analysis Xarray Package Background Estimation
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