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Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac
 

Quantification of Endogenous Auxin and Cytokinin During Internode Culture of Ipecac

Article DOI: 10.3791/56902-v • 07:57 min • March 15th, 2018
March 15th, 2018 •
1, 1, 1
1Graduate School of Life Sciences, Toyo University

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Adventitious shoots can be induced on internodal segments of ipecac without phytohormone treatment. To evaluate phytohormone dynamics during adventitious shoot formation, we measured endogenous auxin and cytokinin in internodal segments by LC-MS/MS.

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Keywords: Ipecac Internode Culture Endogenous Auxin Endogenous Cytokinin Phytohormone Quantification Adventitious Shoot Formation Tissue Regeneration B5 Medium Zirconia Bead Acetonitrile Extraction HPLC-MS/MS
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