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JoVE Journal Developmental Biology
Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development
 

Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

Article DOI: 10.3791/57068-v • 10:30 min • June 12th, 2018
June 12th, 2018 •
1,3, 2, 2, 1,3
1Faculty of Life and Environmental Sciences, Department of Biotechnology, University of Yamanashi, 2Department of Integrated Bioscience, Graduate School of Frontier Sciences, University of Tokyo, 3Advanced Biotechnology Center, University of Yamanashi

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Chromatin looseness appears to be involved in the developmental potential of blastomeres. However, it is not known whether chromatin looseness can be used as a reliable index for the developmental potential for embryos. Here, an experimental system in which chromatin looseness-evaluated zygotes can develop to full term has been described.

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Zygotic FRAP ZFRAP Chromatin Looseness Full-term Development Embryonic Development Developmental Biology Technique Visual Demonstration EGFP-H2B RNA Dilution Dish PVP-HTF Hepes-buffered CZB Mineral Oil Pipette Puller Microforge Glass Pipette Microinjection MRNA Injection Fertilized Zygotes
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