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JoVE Journal Biochemistry
Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
 

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Article DOI: 10.3791/57177-v • 09:33 min • March 20th, 2018
March 20th, 2018 •
1
1Department of Biochemistry, Charles University

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Evaluating the potency of environmental chemicals and drugs, to be enzymatically bioactivated to intermediates generating covalent DNA adducts, is an important field in the development of cancer and its treatment. Methods are described for compound activation to form DNA adducts, as well as techniques for their detection and quantification.

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Keywords: Covalent DNA Adducts Enzymatic Activation Carcinogens Drugs 32P-postlabeling Cytochrome P450 Biotransformation Cancer Development Molecular Mechanisms DNA Damage Toxification
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