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Immunology and Infection

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JoVE Journal Immunology and Infection
Using Terminal Transferase-mediated dUTP Nick End-labelling (TUNEL) and Caspase 3/7 Assays to Measure Epidermal Cell Death in Frogs with Chytridiomycosis
 

Using Terminal Transferase-mediated dUTP Nick End-labelling (TUNEL) and Caspase 3/7 Assays to Measure Epidermal Cell Death in Frogs with Chytridiomycosis

Article DOI: 10.3791/57345-v • 07:37 min • May 16th, 2018
May 16th, 2018 •
1,2, 1, 1, 1
1One Health Research Group, College of Public Health, Medical and Veterinary Sciences, James Cook University, 2Department of Biological Sciences, University of Pittsburgh

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We quantify epidermal cell death in frogs with chytridiomycosis using two methods. First, we use terminal transferase-mediated dUTP nick end-labelling (TUNEL) in situ histology to determine differences between clinically infected and uninfected animals. Second, we conduct a time series analysis of apoptosis over infection using a caspase 3/7 protein analysis.

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Terminal Transferase-mediated DUTP Nick End-labelling (TUNEL) Caspase 3/7 Assays Epidermal Cell Death Frogs Chytridiomycosis Amphibian Fungal Pathogen Batrachochytrium Dendrobatidis Apoptosis Cell Death Epidermal Tissue Wildlife Disease Infection Immune Mechanisms Hydrophilic Glass Slides Tissue Biopsy Samples Deparaffinize Xylene Ethanol PBS Proteinase K Positive Control Slides DN Buffer
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