Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Rapid In Vivo Assessment of Adjuvant's Cytotoxic…
JoVE Journal
Medicine

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Medicine
Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
 

Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

Article DOI: 10.3791/57401-v • 09:03 min • June 19th, 2018
June 19th, 2018 •
1, 1, 1, 1, 1, 1, 1, 1
1Department of Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research

Capítulos

Resumo
Automatic Translation

We present here an application for a standard immunological technique (CFSE stained OT-I proliferation) intended to rapidly monitor adjuvant-mediated cytotoxic T lymphocyte (CTL) generation in vivo. This fast estimation of CTL capacities is useful for the development of prophylactic vaccines against intracellular pathogens as well as therapeutic cancer vaccines.

Tags

Keywords: OT1 CD8 T Cells CFSE Adoptive Transfer Adjuvant Cytotoxic T Lymphocyte (CTL) Response Vaccine Development Lymph Node Spleen Magnetic Bead Isolation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter