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JoVE Journal Cancer Research
Desthiobiotin-streptividin-affinitet medierad rening av RNA-interagera proteiner i mesoteliom celler
 
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Desthiobiotin-streptividin-affinitet medierad rening av RNA-interagera proteiner i mesoteliom celler

Article DOI: 10.3791/57516-v • 11:11 min • April 25th, 2018
April 25th, 2018 •
1, 1
1Laboratory of Molecular Oncology, Division of Thoracic Surgery, University Hospital Zurich

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Desthiobiotin märkning av en syntetisk 25-nukleotid RNA oligo, som innehåller en adenin-rika element (är) motiv, tillåter specifika bindningen av cytosoliska är-bindande protein.

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Cancerforskning fråga 134 RNA-bindande protein RNA-pulldown icke-kodande RNA 3' UTR mesoteliom mänskliga-antigen R
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