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JoVE Journal Developmental Biology
Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs
 

Three-dimensional Organotypic Cultures of Vestibular and Auditory Sensory Organs

Article DOI: 10.3791/57527-v • 08:17 min • June 1st, 2018
June 1st, 2018 •
1,2, 3, 1,2
1Department of Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of the University of Southern California, 2Caruso Department of Otolaryngology-Head and Neck Surgery, Keck School of Medicine of the University of Southern California, 3Howard Hughes Medical Institute and Laboratory of Sensory Neuroscience, The Rockefeller University

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Resumo
Automatic Translation

Three-dimensional organotypic cultures of the murine utricle and cochlea in optically clear collagen I gels preserve innate tissue morphology, allow for mechanical stimulation through adjustment of matrix stiffness, and permit virus-mediated gene delivery.

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Keywords: 3D Organotypic Culture Vestibular Organ Auditory Organ Inner Ear Cochlea Utricle Sensory Organ Development Mechanical Force Tissue Stiffness In Vitro Model
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