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Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice
 

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Article DOI: 10.3791/57560-v • 09:00 min • August 2nd, 2018
August 2nd, 2018 •
1, 1, 1, 1
1Department of Surgery, Davis Heart and Lung Research Institute, Biomedical Sciences Graduate Program, Biophysics Graduate Program, Ohio State University Wexner Medical Center

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Here we provide a detailed protocol to carry out in vivo cardiac gene editing in mice using recombinant Adeno-Associated Virus(rAAV)-mediated delivery of CRISPR. This protocol offers a promising therapeutic strategy to treat dystrophic cardiomyopathy in Duchenne muscular dystrophy and can be used to generate cardiac-specific knockout in postnatal mice.

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Keywords: Adeno-associated Virus CRISPR Cardiac Gene Editing Muscular Dystrophy Dystrophin Duchenne's Muscular Dystrophy Gene Editing Gene Therapy GRNA Design CRISPR Vector Ligation
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