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In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
 

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Article DOI: 10.3791/57845-v • 09:53 min • June 15th, 2018
June 15th, 2018 •
1, 2, 2
1Princess Margaret Cancer Centre, University of Toronto, 2Department of Pathology, Department of Cardiology, Johns Hopkins University

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Resumo
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Tissue-specific microRNA inhibition is a technology that is underdeveloped in the microRNA field. Herein, we describe a protocol to successfully inhibit the miR-181 microRNA family in myoblast cells from the heart. Nanovector technology is used to deliver a microRNA sponge that demonstrates significant in vivo cardio-specific miR-181 family inhibition.

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Keywords: In Vivo Nanovector MicroRNA Sponge Heart-specific Tissue-specific MicroRNA Knockdown EGFR Plasmid CMV Promoter Alpha-myosin Heavy Chain Promoter DNA Purification Bacterial Transformation Ligation
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