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JoVE Journal Biochemistry
Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
 

Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling

Article DOI: 10.3791/57901-v • 10:27 min • October 21st, 2018
October 21st, 2018 •
1, 2, 1, 1, 2, 1
1Neutron Scattering Division, Oak Ridge National Laboratory, 2School of Chemistry and Biochemistry, Georgia Institute of Technology

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Resumo
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This protocol demonstrates how to obtain a low-resolution ab initio model and structural details of a detergent-solubilized membrane protein in solution using small-angle neutron scattering with contrast-matching of the detergent.

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Contrast-matching Detergent Small-angle Neutron Scattering Membrane Protein Structural Analysis Ab Initio Modeling Integral Membrane Proteins Oligomeric State Overall Shape Low Resolution Structure Solution Studies Stabilizing Molecules Invisible Detergents Signal From Membrane Protein Biological Research Medical Research Deuterium Labeling Neutron Scattering Length Densities SLDs Contrast Variation Data Analysis MULCh Web Application Project Title Subunit One Subunit Two Molecular Formula Volume In Cubic Angstroms Buffer Components Neutron Contrast Calculation
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