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Imaging FITC-dextran as a Reporter for Regulated Exocytosis
 

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

Article DOI: 10.3791/57936-v • 04:50 min • June 20th, 2018
June 20th, 2018 •
1, 1, 2, 3, 4, 1
1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Department of Pathology, Sackler Faculty of Medicine, Tel Aviv University, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University, 4Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine, Stanford University

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Here we detail a method for live cell imaging of regulated exocytosis. This method utilizes FITC-dextran, which accumulates in lysosome-related organelles, as a reporter. This simple method also allows distinguishing between different modes of regulated exocytosis in cells that are difficult to manipulate genetically.

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Keywords: FITC-dextran Regulated Exocytosis Mast Cell Exocytosis Neutrophils Eosinophils Tyrode's Buffer RBL Cells Ammonium Chloride Secretagogue Fluorescence Microscopy
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