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JoVE Journal Developmental Biology
Caenorhabditis Sieve
 

Caenorhabditis Sieve: A Low-tech Instrument and Methodology for Sorting Small Multicellular Organisms

Article DOI: 10.3791/58014-v • 04:44 min • July 4th, 2018
July 4th, 2018 •
*1,2, *3, 4, 2, 5
1Department of Biology and Wildlife, University of Alaska Fairbanks, 2Institute of Arctic Biology, University of Alaska Fairbanks, 3Department of Chemistry and Biochemistry, University of Alaska Fairbanks, 4Departments of Biology and Chemistry, Earlham College, 5Department of Biological Sciences, College of Natural Science and Mathematics, California State University Long Beach
* These authors contributed equally

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The current protocol includes a methodology for the sorting and cleaning of age-matched populations of Caenorhabditis elegans. It uses a simple, inexpensive, and efficient custom-made tool to obtain a large experimental population of nematodes for research.

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Caenorhabditis Sieve Low-tech Instrument Sorting Methodology Small Multicellular Organisms C. Elegans Molecular Biology Gene Expression Assays Protein Expression Assays Developmental Stages Body Size Selection Time And Cost Efficiency Arching Turnover Step Solution Flow Visual Demonstration Washing Step Flipping Step 50 ML Conical Tubes Stepped Drill Bit Nylon Monofilament Woven Mesh Cyanoacrylate Glue
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