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Immunology and Infection

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JoVE Journal Immunology and Infection
A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion
 

A High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion

Article DOI: 10.3791/58074-v • 07:22 min • August 14th, 2018
August 14th, 2018 •
1,2, 1, 1, 1, 1, 1
1Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, Immunology Institute, 2Department of Biology, New Jersey City University

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We describe a cell-based assay to report on HIV-1 fusion via the expression of green fluorescent protein detectable by flow cytometry or fluorescence microscopy. It can be used to test inhibitors of viral entry (specifically at the fusion step) in cell-free and cell-to-cell infection systems.

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Keywords: Cre-Lox Viral Membrane Fusion HIV-1 Drug Discovery High-throughput Screening HIV Fusion Inhibitors Biosafety Jurkat RG Reporter Cells Puromycin Cell-to-cell Virus Transmission Gag-iCre DNA Electroporation Ficoll
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