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Journal / Immunology and Infection / 小鼠骨髓基质树突状细胞诱导 CD4 T 细胞活化、增殖和 Th1…
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Immunology and Infection

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JoVE Journal Immunology and Infection
小鼠骨髓基质树突状细胞诱导 CD4 T 细胞活化、增殖和 Th1 分化的体外模型研究
 
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小鼠骨髓基质树突状细胞诱导 CD4 T 细胞活化、增殖和 Th1 分化的体外模型研究

Article DOI: 10.3791/58118-v • 08:39 min • August 22nd, 2018
August 22nd, 2018 •
1, 1, 2, 1,2,3
1LamImSys Lab, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 2LamImSys Lab, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 3CIBER de Enfermedades Cardiovasculares

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在这里, 我们提出了一种在体内测定由 GM-脑脊液骨髓 (BM) 衍生树突状细胞 (CD4) 诱导的天真的 t 细胞 (t 细胞) 活化、增殖和 Th1 分化的方法。此外, 该协议还描述了 BM 和 t 细胞分离、dc 生成和 dc 和 t 细胞的移植。

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免疫学和感染 问题 138 免疫学 CD4 t 细胞 t 细胞活化 t 细胞增殖 t 细胞分化 骨髓源性树突状细胞 领养细胞移植 小鼠 骨髓分化 OTII 磁性细胞分离
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