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Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis
 

Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis

Article DOI: 10.3791/58358-v • 08:16 min • February 26th, 2019
February 26th, 2019 •
1, 2, 2, 1,3,4, 1
1Instituto de Investigación Sanitaria, 2Unidad de Animalario del Centro de Investigación Príncipe Felipe (CIPF), 3Departamento de Pediatría, Obstetricia y Ginecología, Universidad de Valencia, 4Servicio de Obstetricia y Ginecología, Hospital Clínico Universitario de Valencia

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Here, we present a protocol for live-imaging of fluorescently labeled human endometrial fragments grafted in mice. The method allows studying the effects of drugs of choice on endometriotic lesion size through monitoring and quantification of fluorescence emitted by the fluorescent reporter on real time

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Noninvasive Monitoring Lesion Size Heterologous Mouse Model Endometriosis Therapeutic Drug Effects Anti-androgenic Compounds Animal Models Real-time Assessment Jessica Martinez Technician Viviana Bisbal Nerea Marin Veterinarians Centro De Investigacion Principe Felipe Collaborators Implant Surgery Endometrial Tissue Biopsy Fragments Petri Dish Complete Medium Scalpels Mince Tissue Micropipette Drops Of DMEM Culture Dish Syringe Needle Biopsy Tissue Fragment Adeno-mCherry 96 Well Plate
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