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Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model
 

Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model

Article DOI: 10.3791/62410-v • 10:32 min • April 2nd, 2021
April 2nd, 2021 •
1, 1
1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University

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This protocol describes a detailed method for the preparation and immunofluorescence staining of mice retinal flat mounts and analysis. The use of fluorescein fundus angiography (FFA) for mice pups and image processing are described in detail as well.

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Keywords: Retinal Vessels Oxygen-induced Retinopathy Mouse Model Retinal Whole Mounting Retinal Vasculature Retinal Separation Eye Dissection Tissue Collection Tissue Fixation
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