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使用 NETQUANT 对中性粒细胞外陷阱的自动基于图像的定量

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT

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使用 NETQUANT 对中性粒细胞外陷阱的自动基于图像的定量

DOI:

10.3791/58528-v

•

07:33 min

•

November 27, 2019

•

Tirthankar Mohanty, Pontus Nordenfelt

¹Department of Clinical Sciences, Division of Infection Medicine,Lund University

Capítulos

00:04Título
00:34Analysis and Quantification of Neutrophil Extracellular Traps (NETs)
02:19Segmentation of Cells in the DNA Channel and the NET Channel
03:41Single-cell Analysis of Identifiable Properties
04:19Comparison of Cell Properties to Identify NET-forming Cells
05:23Result Outputs Demeanor
06:16Results: Analysis of NET Formation
07:07Conclusion

Summary

Tadução automática

在这里，我们提出了一个协议，用于生成中性粒细胞外陷阱（NETs）和操作NETQUANT，这是一种用于定量免疫荧光图像中的NET的全自动软件选项。

Tags

Keywords: NETQUANT Automated Image Analysis Neutrophil Extracellular Traps (NETs)Immunofluorescence Imaging Image Segmentation Adaptive Segmentation DNA Channel NET Channel Sample Types Batch Processing Image Metadata Zenodo Nordonfelt Lab

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