Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Isolation of Glomeruli and In Vivo Labeling of…
JoVE Journal
Biochemistry

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Biochemistry
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
 

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Article DOI: 10.3791/58542-v • 09:12 min • January 18th, 2019
January 18th, 2019 •
1, 1, 1, 2, 1, 1, 1, 1, 1, 1
1Department of Nephrology, Medical Faculty, Heinrich-Heine-University, 2Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf

Capítulos

Resumo
Automatic Translation

Here we present a protocol for murine in vivo labeling of glomerular cell surface proteins with biotin. This protocol contains information on how to perfuse mouse kidneys, isolate glomeruli, and perform endogenous immunoprecipitation of the protein of interest.

Tags

Keywords: Glomeruli Isolation In Vivo Labeling Glomerular Cell Surface Proteins Proteinuric Kidney Disease Cell Surface Protein Trafficking Organ Perfusion Kidney Isolation Aortic Perfusion Renal Vein PBS Perfusion
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter