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JoVE Journal Biochemistry
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
 

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Article DOI: 10.3791/58952-v • 08:59 min • February 12th, 2019
February 12th, 2019 •
1,2, 1, 1, 1, 1, 1
1Cellular Protein Chemistry, Utrecht University, 2Program in Cellular and Molecular Medicine, Boston Children's Hospital

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Here we describe a protocol for a general pulse-chase method that allows the kinetic analysis of folding, transport, and degradation of proteins to be followed in live cells.

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Protein Folding Protein Transport Protein Degradation Radioactive Pulse Chase 35S-labeled Methionine 35S-labeled Cysteine Protein Biosynthesis Translation Folding And Assembly Trafficking And Degradation Radioactive Workspace Buffers Clear Schedule Microscopy Inspection Wash Buffer Starvation Medium Humidified Incubator Carbon Dioxide Water Bath
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