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JoVE Journal Neuroscience
Ein Edelstahl-Protokoll für hochwertige RNA Isolation aus Laser Capture Microdissected Purkinje-Zellen im menschlichen Post-Mortem Kleinhirn
 
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Ein Edelstahl-Protokoll für hochwertige RNA Isolation aus Laser Capture Microdissected Purkinje-Zellen im menschlichen Post-Mortem Kleinhirn

Article DOI: 10.3791/58953-v • 09:04 min • January 17th, 2019
January 17th, 2019 •
1, 2,3,4, 1
1Department of Pathology and Cell Biology, Columbia University, 2Division of Movement Disorders, Department of Neurology, Yale University, 3Department of Chronic Disease Epidemiology, Yale School of Public Health, Yale University, 4Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine, Yale University

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Dieses Protokoll verwendet einen fleckenfreie Ansatz zu visualisieren und Purkinje-Zellen im Tiefkühlfrische Gewebe von Post-Mortem-menschlichen Kleinhirn per Laser Capture Microdissection zu isolieren. Der Zweck dieses Protokolls ist, ausreichende Mengen an qualitativ hochwertigen RNA für die RNA-Sequenzierung zu generieren.

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Neurowissenschaften Ausgabe 143 Purkinje Zelle Kleinhirn laser Capture Microdissection RNA RNAseq essentieller Tremor
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