Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Chemistry / Synthesis and Bioconjugation of Thiol-Reactive…
JoVE Journal
Chemistry

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Chemistry
Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates
 

Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

Article DOI: 10.3791/59063-v • 08:47 min • March 6th, 2019
March 6th, 2019 •
1, 1,2, 1, 1,2,3,4
1Department of Chemistry, Hunter College of the City University of New York, 2Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 3Department of Radiology, Memorial Sloan Kettering Cancer Center, 4Department of Radiology, Weill Cornell Medical College

Capítulos

Resumo
Automatic Translation

In this protocol, we will describe the synthesis of PODS, a phenyoxadiazolyl methyl sulfone-based reagent for the site-selective attachment of cargos to the thiols of biomolecules, particularly antibodies. In addition, we will describe the synthesis and characterization of a PODS-bearing bifunctional chelator and its conjugation to a model antibody.

Tags

Keywords: Thiol-reactive Reagents Site-specific Bioconjugation Immunoconjugates PODS Maleimide-thiol-based Bioconjugation Aminophenyl Oxadiazole Thiol Diisopropylethylamine Iodomethane Boc-protected Carboxylic Acid Dichloromethane EDCI Aniline Hydrochloric Acid Sodium Carbonate Magnesium Sulfate Ethyl Acetate Cyclohexane Carbamate M-chloroperbenzoic Acid
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter