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Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta
 

Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta

Article DOI: 10.3791/59153-v • 05:56 min • May 15th, 2019
May 15th, 2019 •
1, 2, 2, 1
1Invasive Insect Biocontrol and Behavior Lab, United States Department of Agriculture (USDA), Agricultural Research Service (ARS), 2Genetic Improvement for Fruits and Vegetables Lab, USDA-ARS

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The presented method creates natural herbivore damaged plant tissue through the application of Manduca sexta larvae to detached leaves of potato. The plant tissue is assayed for expression of six transcription factor homologs involved in early responses to insect herbivory.

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Keywords: Detached Leaf Assay Gene Expression Potato Manduca Sexta Insect Herbivory Chewing Insect Tissue Culture Nodal Propagation Larval Stage Plant-insect Interaction Reproducible Data Plant Genetic Improvement
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