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Bioengineering

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JoVE Journal Bioengineering
Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
 

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Article DOI: 10.3791/59177-v • 09:30 min • May 24th, 2019
May 24th, 2019 •
1,2, 2,3, 4, 2,3, 2,3
1Department of Integrated Traditional Chinese and Western Medicine, West China Hospital of Sichuan University, 2Virginia G. Piper Biodesign Center for Personalized Diagnostics, The Biodesign Institute, Arizona State University, 3School of Biological and Health Systems Engineering, Arizona State University, 4Department of Electrical and Computer Engineering, North Dakota State University

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Clinical translation of exosome-derived biomarkers for diseased and malignant cells is hindered by the lack of rapid and accurate quantification methods. This report describes the use of low-magnification dark-field microscope images to quantify specific exosome subtypes in small volume serum or plasma samples.

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Nanoplasmon-enhanced Scattering NPES Low-magnification Microscope Imaging Tumor-derived Exosomes Exosome Analysis Biomarkers Clinical Sample Genetically Engineered Models PDX Mouse Models NeutrAvidin-functionalized Gold Nanorods Cold PBS Biotinylated Antibodies
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