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JoVE Journal Neuroscience
A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain
 

A Human Blood-Brain Interface Model to Study Barrier Crossings by Pathogens or Medicines and Their Interactions with the Brain

Article DOI: 10.3791/59220-v • 07:52 min • April 9th, 2019
April 9th, 2019 •
*1, *1, 1, 2, 2, 3, 1
1Institut Pasteur, CNRS UMR 3569, Unité de Neuroimmunologie Virale, 2Institut Pasteur, Unité d’Epidémiologie et de Pathophysiologie des Virus Oncogènes, Université Sorbonne Paris Cité/Paris Diderot, 3Institut Pasteur, PFIA, DTPS
* These authors contributed equally

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Resumo
Automatic Translation

Here we present a protocol describing the setting of an in cellulo BBB (Blood brain barrier)-Minibrain polyester porous membrane culture insert system in order to evaluate the transport of biomolecules or infectious agents across a human BBB and their physiological impact on the neighboring brain cells.

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Keywords: Blood-brain Interface Blood-brain Barrier BBB Pathogen Medicine Neurosystem Mini-brain Endothelial Cells Lucifer Yellow Permeability Transport Buffer CHME Clone Five Cells Neurons Astrocytes Polyester Membrane Cell Culture Cell Seeding Cell Counting Cell Trypsinization
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