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An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells
 

An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells

Article DOI: 10.3791/59419-v • 04:37 min • December 16th, 2019
December 16th, 2019 •
1,2, 3, 1, 3, 1,2
1Department of Medicine, Division of Hematology/Oncology, New Jersey Medical School, Rutgers Biomedical and Health Sciences, 2Rutgers School of Graduate Studies at New Jersey Medical School, Rutgers Biomedical and Health Sciences, 3Department of Surgery, Division of Plastic Surgery, New Jersey Medical School, Rutgers Biomedical and Health Sciences

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This protocol provides an enzyme-free method for isolating mesenchymal stem cells from abdominoplasty and lipoaspirate samples using an explant method. The absence of harsh enzymes or centrifugation steps provides for clinically relevant stem cells that can be used for studies in vitro or transferred back to the clinic.

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Keywords: Adipose-derived Mesenchymal Stem Cells Enzyme-free Isolation Explant Method Tissue Mincing Cell Expansion Tissue Culture Trypsinization Clinical Applications Research Applications
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