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Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats
 

Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats

Article DOI: 10.3791/61532-v • 08:58 min • August 25th, 2020
August 25th, 2020 •
*1, *1, 1, 1, 1, 1, 2, 1
1Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology, 2The 2nd Dental Center, Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, National Clinical Research center of Stomatology
* These authors contributed equally

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This article presents a method that combines whole bone marrow adherence and flow cytometry sorting for isolating, cultivating, sorting, and identifying bone marrow mesenchymal stem cells from rat mandibles.

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Keywords: Mandibular Bone Marrow Mesenchymal Stem Cells Isolation Cultivation Rats Cranial Facial Bone BMSCs Bone Marrow Adherence Fluorescent Cell Sorting Mandibular Body Mandibular Ramus Coracoid Condyle Marrow Cavity Alpha-MEM FBS Centrifuge
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