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JoVE Journal Developmental Biology
Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films
 

Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films

Article DOI: 10.3791/59855-v • 04:14 min • June 19th, 2019
June 19th, 2019 •
*1, *2, 1, 1, 1, 3, 1
1Department of Periodontology, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, 2Department of Periodontology, Dental Diseases Prevention & Treatment Center of Jiading District, 3College of Materials Science and Engineering, Tongji University
* These authors contributed equally

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Resumo
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Here, we present protocols of culturing human periodontal ligament (PDL) cell spheroids by chitosan films. The culture of three-dimensional (3D) cellular spheroids provides an alternative to conventional tissue culture polystyrene (TCPS) culture system.

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Keywords: Periodontal Ligament Cells Cell Spheroids Chitosan Films Cell Culture Osteogenic Differentiation Cell Seeding Density Cell Morphology
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