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JoVE Journal Genetics
A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
 

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Article DOI: 10.3791/59901-v • 09:29 min • August 21st, 2019
August 21st, 2019 •
1, 1, 1
1Division of Medical Genetics, University of Washington School of Medicine

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Here, we describe a step-by-step strategy for isolating small RNAs, enriching for microRNAs, and preparing samples for high-throughput sequencing. We then describe how to process sequence reads and align them to microRNAs, using open source tools.

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Keywords: MicroRNA RNA Sequencing Bioinformatics Gel Purification Small RNA RNA Isolation Gene Therapy Disease Mechanism
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