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Ex vivo oculomotor skive kultur fra embryonale GFP-udtrykker mus for tidsforskudt billeddannelse af oculomotor nerve outgrowth
 
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Ex vivo oculomotor skive kultur fra embryonale GFP-udtrykker mus for tidsforskudt billeddannelse af oculomotor nerve outgrowth

Article DOI: 10.3791/59911-v • 06:04 min • July 16th, 2019
July 16th, 2019 •
1,2,3, 1,3, 1,3, 1,2,3,4,5,6
1Department of Ophthalmology, Boston Children's Hospital, 2Department of Ophthalmology, Harvard Medical School, 3F.M. Kirby Neurobiology Center, Boston Children's Hospital, 4Department of Neurology, Boston Children's Hospital, 5Department of Neurology, Harvard Medical School, 6Howard Hughes Medical Institute

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En ex vivo skive analyse tillader oculomotoriske nerve udvækst at blive afbildet i realtid. Udsnit genereres ved at indlejre E 10.5 ISLMN: gfp -embryoner i agopstået, udskæring på en vibratome og vokser i en scene-top inkubator. Axon-vejlednings forløbene vurderes ved at tilføje inhibitorer til kultur medierne.

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Neurovidenskab oculomotor medfødt kranie dysinnervationforstyrrelse Axon-vejledning øjenbevægelser tidsforskudt billeddannelse Skive kultur
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