Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Identifikation af fodspor af RNA: protein…
JoVE Journal
Genetics

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Genetics
Identifikation af fodspor af RNA
 
Click here for the English version

Identifikation af fodspor af RNA: protein komplekser via RNA Immunopræcipitation i tandem efterfulgt af sekventering (RIPiT-SEQ)

Article DOI: 10.3791/59913-v • 09:26 min • July 10th, 2019
July 10th, 2019 •
1, 1, 1
1Department of Molecular Genetics, Center for RNA Biology, The Ohio State University

Capítulos

Resumo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Her præsenterer vi en protokol for at berige endogene RNA-bindingssteder eller "fodspor" af RNA: protein (RNP)-komplekser fra pattedyrsceller. Denne fremgangsmåde involverer to immunoprecipitationer af RNP under enheder og er derfor døbt RNA chromatinimmunpræcipitation i tandem (ripit).

Tags

Genetik RNA-fodaftryk RIPiT RNA-bindingsprotein RBP RNA: protein komplekser RNP
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter