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JoVE Journal Genetics
Identification of Circular RNAs using RNA Sequencing
 

Identification of Circular RNAs using RNA Sequencing

Article DOI: 10.3791/59981-v • 08:25 min • November 14th, 2019
November 14th, 2019 •
1,2, 1,2, 1,2, 1,2, 2,3, 2,3, 1,2
1Translational Genomic Research Institute, 2Arizona Alzheimer's Consortium, 3Banner Sun Health Research Institute

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Circular RNAs (circRNAs) are non-coding RNAs that may have roles in transcriptional regulation and mediating interactions between proteins. Following assessment of different parameters for construction of circRNA sequencing libraries, a protocol was compiled utilizing stranded total RNA library preparation with RNase R pre-treatment and is presented here.

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Identification Circular RNAs RNA Sequencing CircRNA Library Preparation Kit Type RNase R Treatment Input Amounts CircRNA Detection Adjustable Parameters Sample Types Expression Distribution Functional Role Total RNA Sample RIN Value DV200 Value Agilent Bioanalyzer TapeStation Diluting Total RNA RNase-free Water RNase R Buffer Working Concentration Reaction Tube Diluted RNase R Pipetting Up And Down 37-degree Celsius Water Bath
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