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JoVE Journal Developmental Biology
En Ectopic chemokine udtryks model til afprøvning af makrofag rekruttering in vivo
 
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En Ectopic chemokine udtryks model til afprøvning af makrofag rekruttering in vivo

Article DOI: 10.3791/60161-v • 10:02 min • September 25th, 2019
September 25th, 2019 •
1, 1, 2
1Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, 2Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology

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For at afprøve virkningen af en kemokin på makrofag rekruttering in vivo, blev hele Mount in situ hybridisering anvendt til at detektere ektopisk ekspression af chemokin, og immun farvning blev brugt til at mærke makrofager. Live imaging blev brugt til realtids observation af makrofag migration.

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Udviklingsmæssige biologi hele Mount fluorescerende in situ hybridisering immun farvning levende billeddannelse zebrafish Il-34 makrofag
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