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JoVE Journal Cancer Research
Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
 

Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures

Article DOI: 10.3791/60357-v • 09:06 min • December 13th, 2019
December 13th, 2019 •
1, 1, 1, 1, 1,2,3
1Department of Urology, College of Medicine, University of Illinois at Chicago, 2Department of Pathology, College of Medicine, University of Illinois at Chicago, 3University of Illinois Cancer Center, College of Medicine, University of Illinois at Chicago

Capítulos

Resumo
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Using human primary prostate epithelial cells, we report a novel biomarker-free method of functional characterization of stem-like cells by a spheroid-based label-retention assay. A step-by-step protocol is described for BrdU, CFSE, or Far Red 2D cell labeling; three-dimensional spheroid formation; label-retaining stem-like cell identification by immunocytochemistry; and isolation by FACS.

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Keywords: Stem Cells Spheroid Culture Label-retention Assay CFSE Far Red Prostate Cancer Epithelial Cells 3D Culture Cell Isolation Therapeutic Resistance Cancer Stem Cells
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