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JoVE Journal Biochemistry
Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
 

Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations

Article DOI: 10.3791/60402-v • 10:26 min • November 7th, 2019
November 7th, 2019 •
1, 1, 1, 1, 1, 1, 1, 1
1Aix-Marseille Univ, INSERM, CNRS, Institut Paoli-Calmettes, CRCM

Capítulos

Resumo
Automatic Translation

This protocol aims at establishing ubiquitin (Ub) and ubiquitin-likes (Ubls) specific proteomes in order to identify alterations of these kind of post-translational modifications (PTMs), associated with a specific condition such as a treatment or a phenotype.

Tags

Keywords: Ubiquitin Ubiquitin-like Post-translational Modifications Profiling Lentivirus HEK 293T Cells Transfection Protein Purification Eukaryotic Models Diagnosis Prognosis Molecular Targets
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