JoVE Journal
Immunology and Infection
Immunology and Infection
This Conteúdo is Open Access.
Capítulos
Resumo
Here we describe a robust method of determining immune cell identity and purity through epigenetic signatures detected using quantitative PCR (qPCR). DNA demethylation at a specific locus serves as a unique identifier for a particular cell type and allows for identification of CD8+, regulatory, or Th17 T cells.