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Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry
 

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Article DOI: 10.3791/60475-v • 07:57 min • December 26th, 2019
December 26th, 2019 •
*1,2, 3, 1,3, 1, 2,4, *1
1Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne, University of Lausanne, 2Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), 3Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois, 4Hematology Service, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV)
* These authors contributed equally

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Here we describe a reliable method to measure mitochondrial mass and membrane potential in ex vivo cultured hematopoietic stem cells and T cells.

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Mitochondrial Mass Mitochondrial Membrane Potential Flow Cytometry Hematopoietic Stem Cells T-cells Metabolic Assays Rare Populations Mitochondrial Membrane Profiling Metabolic Modulators HSCs Functional Assays Bone Marrow Transplantation Colony-forming Assays Immune T Cells Pump Inhibitor Verapamil Ionic Equilibrium
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