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JoVE Journal Bioengineering
Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay
 

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay

Article DOI: 10.3791/60899-v • 05:16 min • July 26th, 2021
July 26th, 2021 •
1, 1
1Center for Integrated Nanotechnologies, Sandia National Laboratories

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Resumo
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This protocol describes a process for fabricating lipid nanotube networks using gliding kinesin motility in conjunction with giant unilamellar lipid vesicles.

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Lipid Nanotube Networks Gliding Kinesin Motility Assay Phase Separation Behavior Molecular Motors Double-sided Tape Strips Glass Slide Cover Slip Kinesin Solution Microtubule Solution Laboratory Wipe Flow Channel Motility Solution Streptavidin Solution GUV Solution AMP-PNP Solution Sealant Inverted Microscope
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