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JoVE Journal Bioengineering
The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton
 

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

Article DOI: 10.3791/64377-v • 08:50 min • March 10th, 2023
March 10th, 2023 •
1, 1, 1, 1,2
1Department of Chemical Engineering, Ben Gurion University of the Negev, 2Department of Chemical Engineering, Ilse Kats Institute for Nanoscale Science and Technology, Ben Gurion University of the Negev

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In this work, an in vitro reconstitution approach is employed to study the poroelasticity of actomyosin gels under controlled conditions. The dynamics of the actomyosin gel and the embedded solvent are quantified, through which the network poroelasticity is demonstrated. We also discuss the experimental challenges, common pitfalls, and relevance to cell cytoskeleton mechanics.

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Mechanics Poroelastic Contractile Actomyosin Networks Model System Cell Cytoskeleton In Vitro Approach Myosin Contractility Fluid Flow Network And Cytosol Velocities Mechanical Properties Dynamically Evolving Systems Rheology Poroelastic Active Materials AFIM Glass Coverslips Piranha Solution Beaker Double Distilled Water Sonication Bath
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