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JoVE Journal Bioengineering
Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles
 

Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

Article DOI: 10.3791/61106-v • 08:13 min • February 27th, 2021
February 27th, 2021 •
1, 1,2, 1, 1, 2,3
1Laboratoire Matière et Systèmes, Complexes MSC, UMR 7057, CNRS & University Paris Diderot, 2Université Sorbonne Paris Nord, Laboratory for Vascular Translational Science, LVTS, INSERM, UMR 1148, 3Services de Biochimie et Médecine Nucléaire, Hôpital Avicenne Assistance Publique-Hôpitaux de Paris

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Resumo
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Iron oxide nanoparticles are synthesized via a nonaqueous sol gel procedure and coated with anionic short molecules or polymer. The use of magnetometry for monitoring the incorporation and biotransformations of magnetic nanoparticles inside human stem cells is demonstrated using a vibrating sample magnetometer (VSM).

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Keywords: Magnetic Nanoparticles Magnetometry Cellular Incorporation Biodegradation Iron Oxide Nanoparticles Synthesis Biomedical Applications Magnetism Contamination Magnetometer Liquid Samples Biological Samples Iron(III) Acetylacetonate Microwave Reactor Neodymium Magnet Hydrochloric Acid Coating Molecules Human Mesenchymal Stem Cells RPMI Medium
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