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JoVE Journal Cancer Research
Assays for Validating Histone Acetyltransferase Inhibitors
 

Assays for Validating Histone Acetyltransferase Inhibitors

Article DOI: 10.3791/61289-v • 09:11 min • August 6th, 2020
August 6th, 2020 •
1, 1
1Department of Anatomy and Cell Biology, and UF Health Cancer Center, University of Florida College of Medicine

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Inhibitors of histone acetyltransferases (HATs, also known as lysine acetyltransferases), such as CBP/p300, are potential therapeutics for treating cancer. However, rigorous methods for validating these inhibitors are needed. Three in vitro methods for validation include HAT assays with recombinant acetyltransferases, immunoblotting for histone acetylation in cell culture, and ChIP-qPCR.

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Keywords: Histone Acetyltransferase HAT Inhibitor Epigenetic Regulation Enzymatic Reaction Histone Acetylation SDS-PAGE Immunoblotting MCF-7 Cells MS275 A-485 Passive Lysis Buffer Chromatin Immunoprecipitation
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