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Immunology and Infection

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JoVE Journal Immunology and Infection
Modelado de la infección por el virus de la hepatitis B en células no hepáticas 293T-NE-3R
 
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Modelado de la infección por el virus de la hepatitis B en células no hepáticas 293T-NE-3R

Article DOI: 10.3791/61414-v • 09:02 min • June 5th, 2020
June 5th, 2020 •
*1,2,3, *1,2,3,4, 1,2,3, 1,2,3,5, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Stem Cell Research Center, Shantou University Medical College, 2The Center for Reproductive Medicine, Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, 4Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 5Department of Nucleic Acid Researches, Genetic Engineering and Biotechnology Research Institute, General Autority - City of Scientific Researches and Technological Applications
* These authors contributed equally

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Este manuscrito describe un protocolo detallado para la infección por el virus de la hepatitis B (VHB) en células 293T de ingeniería novedosa (293T-NE-3NR, expresando NTCP humano, HNF4, RXR y PPAR) y células hepáticas tradicionales (HepG2-NE, expresando NTCP humano).

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Este mes en JoVE Número 160 Infección por VHB modelo HepG2.2.15 HepG-NE 293T-NE-3NR NTCP receptores de hormonas nucleares
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