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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
 
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Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Article DOI: 10.3791/61665-v • 07:43 min • October 6th, 2020
October 6th, 2020 •
*1,2, *1, 1, 3, 1,3
1Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 2College of Agronomy, Anhui Agricultural University, Heifei, China, 3School of Agriculture, Food and Wine, University of Adelaide
* These authors contributed equally

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Diese Arbeit beschreibt den Einsatz der CRISPR-Cas9 Genom-Editing-Technologie, um das endogene Gen OsABCG15 zu knockouten, gefolgt von einem modifizierten Agrobacterium-vermitteltenTransformationsprotokoll, um eine stabile männlich-sterile Linie in Reis zu produzieren.

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In diesem Monat in JoVE Ausgabe 164 Reis CRISPR-Cas9 Agrobacterium,Callus Gewebekultur Genotyp Phänotyp Anther Pollen
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